SnapGene 4.1.6


Productivity Version: 4.1.6 | updated 4 months ago

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About SnapGene 4.1.6 for macOS

SnapGene is a popular molecular biology software that is easier to use than pen and paper. Now every DNA construct made in your lab can be documented in a rich electronic format… and thanks to the free SnapGene Viewer (available for download in related links below), the files can be shared with colleagues around the world.

In-Fusion Cloning

Clontech’s In-Fusion cloning is a remarkably versatile method for creating seamless gene fusions. SnapGene is the first software to simulate this procedure. Just select the DNA fragments that you wish to fuse, and SnapGene will design the primers.

Gibson Assembly

Many researchers are turning to Gibson Assembly to insert fragments into a plasmid without the use of restriction enzymes. The DNA segments to be joined are amplified by PCR to create overlapping ends. SnapGene simplifies the planning of a Gibson Assembly reaction, and automates the primer design.

Restriction Cloning

SnapGene’s unique restriction cloning interface displays the information you need in a clean layout. Planning of cloning procedures has never been simpler. If you already know what you want to do, the cloning simulation will take only a few seconds. If a cloning procedure has a design flaw, the error can be caught and corrected during the simulation.

PCR & Mutagenesis

After you design primers, they can be used to simulate conventional PCR, overlap extension PCR, or mutagenesis. The resulting DNA sequence files are immediately available for further manipulations. Like the restriction cloning interface, the primer-focused interfaces display key information in an intuitive way.

Automatic Documentation

The most amazing thing about SnapGene is that it automatically records the steps in a cloning project. Each time you edit a sequence or simulate cloning or PCR or mutagenesis, the procedure is automatically logged in a graphical history. After simulating the creation of a DNA construct, you can use the history as an experimental protocol. Embedded in the final file are all of the ancestor constructs, each of which can be resurrected as a separate file.

Agarose Gel Electrophoresis

SnapGene uses an advanced algorithm to create realistic agarose gel simulations. Restriction fragments are shown in three formats: a simulated gel, a numerical list, and a sequence map. You can use the simulated gel to plan a diagnostic restriction digest, or to compare an actual gel image with the predicted pattern.

Enzyme Sites

SnapGene highlights restriction sites in a clear way, automatically marking sites blocked by methylation. Simple controls allow you to choose useful enzyme sets such as “Unique Cutters”, or to define custom enzyme sets and preferred suppliers. Informative tooltips provide key data. The Restriction Enzymes window shows detailed properties for hundreds of commercial enzymes.


With SnapGene, annotating features in a DNA sequence is incredibly easy. The SnapGene format matches GenBank standards but adds options such as color, directionality, and segments. Coding sequences are translated so that you can visualize codons, track amino acid numbering, and check reading frames for gene fusions. Features can be imported from another file, or automatically annotated from a customizable list.

What's New
  • New Functionality:
  • Added the ability to press Alt/Opt while mousing over a sequence trace to see all four peak heights.
  • (Requested by Teisha Rowland)
  • Provided additional TaKaRa DNA markers.
  • (Requested by fky19842004)
  • Enhancements:
  • Enabled bidirectional sorting of a collection by Code Number, Date Added, or Date Created by clicking the column buttons.
  • Made horizontal scrolling easier to discover by setting ∞ as the default fixed line width.
  • Fixes:
  • Fixed an issue on Windows where automatically launching the application after running the installer resulted in the settings being stored in the wrong location and administrator file ownership and permissions being used when saving.
  • (Reported by Clay Fuqua)
  • Improved the output when exporting and printing agarose gels, especially while using high-DPI displays.
  • Corrected an erroneous message that was shown when exporting common features.
  • (Reported by Edward Wallace)
  • Ensured that gray sequence colors are visible after saving and re-opening a file.
  • (Reported by Genevieve Tauxe)
  • Fixed a regression to ensure that gaps in aligned sequences are always visibly represented.
  • (Reported by Dustin Rubinstein)
  • Enhanced the importer for CLC Bio files.
  • (Reported by Rabea Wagner)
  • Fixed an issue with opening certain DNA Strider files.
  • (Reported by Michael Nassal)
  • Improved the import of primers from SeqBuilder files.
  • (Reported by Lakxmi Subramanian)
  • Ensured that error messages in the primer dialogs are completely visible.
  • Enhanced the flexibility of controls for adding and importing primers copied to the clipboard.
  • (Requested by Seth Goldman)
  • Prevented a slowdown and potential data omission that could occur when using the same open file for multiple inserts in a manipulation dialog.
  • (Reported by Seth Goldman)
  • Ensured that modifications to open documents are reflected in manipulation dialogs that already have those documents loaded.
  • Prevented duplicate forward slashes from getting into the recent collections list.
  • Improved the default location for saving sequences in an imported archive.
  • Prevented unwanted data from being retrieved from an imported sequence record when including extra features added by NCBI.
  • Blocked accidental toggling of the “File Name” column in a collection list.

System Requirements
  • ​Mac OS X 10.8 or higher
  • 1GB RAM
  • 250MB available disk space

Additional Downloads
Software stored on the MacAppGuru Servers could be discontinued by their publishers and are no longer commercially available. These files are stored for preservation purposes.
SnapGene User Discussion & Reviews

GSL Biotech LLC

Developer of SnapGene Software for Mac & Windows.
More Apps by GSL Biotech LLC

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Date Feb 27 2018
Category Productivity
Platform macOS
Price 2,495
Version 4.1.6
Reviews 0
Rating 0

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